More came to mind:
CSA
Loss of 5/5 rule
Huge fines for using cell phones by hand
HOS rules changes (specifics needed about each change).
CARB
Diesel particulate filters
Age limits on reefers
Age limits on truck engines
EOBR
HAZMAT background checks
Medical info now required to be filed with state
Passports required to cross U.S./Canada border
IAC certification to haul freight to/from airports
Sleep apnea (pending)
Doctors must be in registry to give DOT physicals (affects us indirectly).
New York sticker on truck (recinded, re-enacted)
SMS (relates to CSA)
Toll increases far greater than the rate of inflation.
Biodiesel
Addition of 34 hour reset (HOS change)
Truck and car speed limit changes, up and down, different ways in different states.
Mexico carriers and drivers allowed to operate in U.S.
Pre-employment screening
Anti-idle restrictions
Speed limiters (Ontario and Quebec)
Tire chain law changes and fine increases
EPA requirements on truck manufacturers
Requriement that if you upgrade your old reefer engine for a new CARB-complant one, you cannot sell the old engine but must destroy it instead.
Red light cameras
CDL driver training (in the works)
No texting while driving.
DEF (not a reg but prompted by them)
TWIC
Move-over laws
Tanker endorsement required to haul totes.
ULSD fuel
No parking on freeway ramps (Indiana, other states?)
No smoking in truck stops (Indoor Air laws)
Remove snow from top trucks and trailers (NJ and some others, I think)
NY-registered trucks, add mirrors to front of trucks if you drive in NYC.
Aerodynamic devices added to trailers (SmartWay)